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minimum essential medium (mem) cell culture medium  (Merck & Co)

 
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    Merck & Co minimum essential medium (mem) cell culture medium
    Minimum Essential Medium (Mem) Cell Culture Medium, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minimum essential medium (mem) cell culture medium/product/Merck & Co
    Average 90 stars, based on 1 article reviews
    minimum essential medium (mem) cell culture medium - by Bioz Stars, 2026-03
    90/100 stars

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    Differential expression of ACSL3 in cell lines and its effect on cell proliferation. A. RT-qPCR analysis of ACSL3 mRNA expression in ccRCC cell lines (ACHN, 769P, and 786O) and the normal renal cell line <t>(293T).</t> B. Western blot analysis of ACSL3 protein expression in ccRCC cell lines and the normal renal cell line. C. RT-qPCR analysis of ACSL3 mRNA expression in h-ACSL3 cells and control cells after transfection. D. Western blot analysis of the ACSL3 protein expression levels in h-ACSL3 cells and control cells after transfection. GAPDH was used as an internal control. Error bars represent M ± SD of triplicate experiments. E. CCK-8 assays were performed to detect the cell proliferation rates of h-ACSL3 cells and control cells at 0, 24, 48, 72, and 96 h. F. Clone formation ability and statistical analysis of h-ACSL3 cells and control cells. Error bars represent M ± SD of triplicate experiments. ns, no statistical significance; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
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    Differential expression of ACSL3 in cell lines and its effect on cell proliferation. A. RT-qPCR analysis of ACSL3 mRNA expression in ccRCC cell lines (ACHN, 769P, and 786O) and the normal renal cell line (293T). B. Western blot analysis of ACSL3 protein expression in ccRCC cell lines and the normal renal cell line. C. RT-qPCR analysis of ACSL3 mRNA expression in h-ACSL3 cells and control cells after transfection. D. Western blot analysis of the ACSL3 protein expression levels in h-ACSL3 cells and control cells after transfection. GAPDH was used as an internal control. Error bars represent M ± SD of triplicate experiments. E. CCK-8 assays were performed to detect the cell proliferation rates of h-ACSL3 cells and control cells at 0, 24, 48, 72, and 96 h. F. Clone formation ability and statistical analysis of h-ACSL3 cells and control cells. Error bars represent M ± SD of triplicate experiments. ns, no statistical significance; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Journal: American Journal of Cancer Research

    Article Title: The mechanism of long-chain acyl-CoA synthetase 3 in inhibiting cell proliferation, migration, and invasion in clear cell renal cell carcinoma

    doi:

    Figure Lengend Snippet: Differential expression of ACSL3 in cell lines and its effect on cell proliferation. A. RT-qPCR analysis of ACSL3 mRNA expression in ccRCC cell lines (ACHN, 769P, and 786O) and the normal renal cell line (293T). B. Western blot analysis of ACSL3 protein expression in ccRCC cell lines and the normal renal cell line. C. RT-qPCR analysis of ACSL3 mRNA expression in h-ACSL3 cells and control cells after transfection. D. Western blot analysis of the ACSL3 protein expression levels in h-ACSL3 cells and control cells after transfection. GAPDH was used as an internal control. Error bars represent M ± SD of triplicate experiments. E. CCK-8 assays were performed to detect the cell proliferation rates of h-ACSL3 cells and control cells at 0, 24, 48, 72, and 96 h. F. Clone formation ability and statistical analysis of h-ACSL3 cells and control cells. Error bars represent M ± SD of triplicate experiments. ns, no statistical significance; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Article Snippet: The ccRCC cell lines ACHN, 769P, 786O, renal cell line 293T, cell basic culture medium minimum essential medium (MEM), and 1640 culture medium were purchased from Procell (Wuhan, China).

    Techniques: Quantitative Proteomics, Quantitative RT-PCR, Expressing, Western Blot, Control, Transfection, CCK-8 Assay